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目的通过研究银杏叶提取物(GBE)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响,探讨GBE的脑保护作用及机制。方法 90只新生7 d SD大鼠随机分为假手术组(n=30)、HIBD模型组(n=30)、GBE治疗组(n=30)。模型制造成功后24 h、72 h、168 h三个时间点,采用干湿质量法测定大鼠脑组织含水量,RT-PCR法检测各组大鼠脑MMP-9、TIMP-1 m RNA的表达情况。结果 CBE组神经系统异常症状较HIBD组更轻。HIBD组大鼠脑组织含水量在缺氧缺血后逐渐上升,72 h达到高峰,168 h开始下降。各时间点均高于假手术组(P<0.05)。同时,MMP-9及TMP-1m RNA表达量与脑含水量呈正相关。GBE治疗组明显小于HIBD模型组(P<0.05),高于假手术组(P<0.05)。结论 GBE对HIBD脑损伤的保护作用可能与其调节MMP-9及TIMP-1的表达有关。
Objective To investigate the effects of Ginkgo biloba extract (GBE) on the expression of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) and brain injury in neonatal rats with hypoxic-ischemic brain damage (HIBD) Expression of the impact of GBE brain protection and mechanism. Methods Ninety newborn SD rats were randomly divided into sham operation group (n = 30), HIBD model group (n = 30) and GBE treatment group (n = 30). After 24 h, 72 h, and 168 h after model establishment, the water content in rat brain was determined by wet and dry method. The expressions of MMP-9 and TIMP-1 mRNA in rat brain were detected by RT-PCR Express the situation. Results The neurological abnormalities in CBE group were lighter than those in HIBD group. The water content of brain tissue in HIBD group increased gradually after hypoxia-ischemia, peaked at 72 h, and decreased at 168 h. All time points were higher than sham operation group (P <0.05). Meanwhile, the expression of MMP-9 and TMP-1m RNA was positively correlated with brain water content. GBE treatment group was significantly less than the HIBD model group (P <0.05), higher than the sham operation group (P <0.05). Conclusion The protective effect of GBE on HIBD brain injury may be related to the regulation of MMP-9 and TIMP-1 expression.