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目的检测N-乙酰氨基半乳糖转移酶7(GALNT7)在乳腺癌与正常组织和细胞中的表达,并分析其表达水平与临床病理特征间的关系。方法采用实时定量反转录-聚合酶链反应(RT-QPCR)法检测GALNT7在40对乳腺癌组织和癌旁正常组织中的表达,运用Mann-Whitney法分析乳腺癌组织中GALNT7表达与患者临床病理特征之间的相关性。培养乳腺癌细胞株(MCF-7和MDA-MB-231)和正常乳腺上皮细胞株(MCF-10A)并采用实时荧光定量PCR方法检测GALNT7表达量。结果 GALNT7在乳腺癌组织中的相对表达量为1.09917±0.01756,癌旁正常组织为0.65218±0.07652,两组比较差异有统计学意义(P<0.01),且乳腺癌组织中GALNT7表达量与患者ER受体表达呈负相关(P=0.016),与其他临床病理资料如年龄、肿瘤大小、PR和HER-2受体表达、临床分期及淋巴结转移无相关关系(P>0.05)。GALNT7在乳腺癌细胞系中的表达量高于正常乳腺上皮细胞系(P<0.001),且ER阴性乳腺癌细胞系MDA-MB-231的GALNT7表达量是ER阳性乳腺癌细胞系MCF-7的2.06倍(P<0.05)。结论 GALNT7在乳腺癌组织和细胞株中均高表达,其在乳腺癌组织和细胞系中的表达与ER受体的表达密切相关,GALNT7与乳腺癌的发生发展相关。
Objective To detect the expression of N-acetylgalactosaminyltransferase 7 (GALNT7) in breast cancer and normal tissues and cells, and to analyze the relationship between its expression and clinicopathological features. Methods The expression of GALNT7 in 40 pairs of breast cancer tissues and adjacent normal tissues was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR). Mann-Whitney method was used to analyze the expression of GALNT7 in breast cancer tissues. Correlation between pathological features. Breast cancer cell lines (MCF-7 and MDA-MB-231) and normal breast epithelial cell line (MCF-10A) were cultured and the expression of GALNT7 was detected by real-time fluorescence quantitative PCR. Results The relative expression of GALNT7 in breast cancer tissues was 1.09917 ± 0.01756, and the normal tissue adjacent to cancer was 0.65218 ± 0.07652 (P <0.01). The expression of GALNT7 in breast cancer tissues was similar to that in patients with ER (P = 0.016). There was no correlation between other clinicopathological parameters such as age, tumor size, PR and HER-2 receptor expression, clinical stage and lymph node metastasis (P> 0.05). The expression level of GALNT7 in breast cancer cell lines was higher than that in normal mammary epithelial cells (P <0.001), and the expression level of GALNT7 in ER-negative breast cancer cell line MDA-MB-231 was significantly higher than that in ER-positive breast cancer cell line MCF-7 2.06-fold (P <0.05). Conclusions GALNT7 is highly expressed in breast cancer tissues and cell lines. The expression of GALNT7 in breast cancer tissues and cell lines is closely related to the expression of ER receptor. GALNT7 is associated with the development of breast cancer.