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目的对盐生肉苁蓉药材进行质量控制研究。方法采用显色法和薄层色谱法鉴别药材中的甜菜碱和甘露醇,以苯酚-硫酸显色法测定多糖的含量。结果盐生肉苁蓉药材中甜菜碱和甘露醇的定性鉴别反应均呈阳性;多糖在4～12μg/ml范围内线性关系良好(r=0.999 1),平均回收率101.5%,RSD=2.2%(n=9)。结论该实验方法可行,重复性良好,是全面控制和评价盐生肉苁蓉药材质量的重要补充。
Objective To study the quality control of Salvia miltiorrhiza. Methods The colorimetric method and TLC were used to identify betaine and mannitol, and the content of polysaccharide was determined by phenol-sulfuric acid colorimetry. Results The qualitative identification of betaine and mannitol in Salvia miltiorrhiza was positive. The polysaccharide showed a good linearity (r = 0.999 1) in the range of 4 ~ 12μg / ml with an average recovery of 101.5% and a RSD of 2.2% (n = 9). Conclusion The experimental method is feasible and reproducible, which is an important complement to comprehensively control and evaluate the quality of Salvia miltiorrhiza.