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本研究利用陆地棉×中棉、陆地棉×海岛棉、独秆突变体×海岛棉、海岛棉×中棉杂交，进行胚珠培养，获得种间杂种植株。结果表明：这４个杂交组合均获得一定频率的胚（包括成熟胚和不成熟胚）及成熟胚萌发的苗；同一组合的杂种胚珠，在不同培养基上获得的胚和成熟胚的频率不同；不同组合的杂交胚珠，在同一培养基上培养的结果有明显差异；进行杂种胚珠培养的适宜时期为授粉后４～６ｄ，２ｄ的胚珠培养易产生愈伤组织，成胚率低，８ｄ后幼胚败育或落桃；适合杂种胚珠早期生理条件的培养基为：ＢＴ基本培养基＋ＮＨ４Ｃｌ１０００ｍｇ／ｌ＋ＩＡＡ１５ｍｇ／Ｌ＋ＫＴ０２ｍｇ／Ｌ＋ＧＡ００６ｍｇ／ｌ；氨态氮能提高成胚率；单一的培养基不能满足杂种胚珠的培养需要。
In this study, the cross of interspecific hybrids was obtained by crossing cotton × cotton, cotton × island cotton, single-stalk mutant × sea island cotton, island cotton × cotton. The results showed that all the four hybrid combinations had the same frequency of embryos (including mature and immature embryos) and mature embryos germinated; the same combination of hybrid ovules, embryos and mature embryos obtained in different media frequency The hybrid ovules cultured in the same culture medium had different results. The suitable period for hybrid ovule culture was 4 ~ 6 days after pollination, and ovule culture was easy to produce callus at 2 days after pollination. The embryo formation rate was low. After 8 days Immature embryo abortion or fall peach; hybrid embryo physiological conditions suitable for early culture medium: BT basic medium + NH4Cl1000mg / l + IAA1 5mg / L + KT0 2mg / L + GA0 06mg / l; ammonia nitrogen can increase the rate of embryo formation; single Medium can not meet the needs of hybrid ovule culture.