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目的研究ＨＣＶ Ｅ１蛋白的Ｂ－细胞表位。方法将携带ＨＣＶ Ｅ１基因的重组质粒ｐＱＥ－３０－ ＨＣＶｅ１１８在Ｅ．ｃｏｌｉ Ｍ１５中进行大量诱导、表达；对表达的Ｅ１蛋白进行分离、纯化；再以纯化的蛋白捕获ＨＣＶ感染者血清中能与 ＨＣＶ Ｅ１蛋白特异反应的 ＩｇＧ，以此 ＩｇＧ作为筛选分子，对随机十二肽库进行淘洗，经 ＥＬＩＳＡ筛选噬菌体阳性克隆并测序。结果ＨＣＶ Ｅ１蛋白获得了高纯度的纯化，这种蛋白可特异地与部分丙型肝炎患者血清发生反应。在获得的 １０个模拟表位中， ６、 ２和２个递呈肽分别与 ＨＣＶ Ｅ１蛋白的３２０～３３６ａａ、 ２５１～２６３ａａ和２２５～２４８位ａａ具有最大同源性。结论在Ｅ１蛋白中存在多个Ｂ－细胞表位，３２０－３３６位极可能为ＨＣＶ Ｅ１蛋白的优势表位。
Objective To study the B-cell epitope of HCV El protein. Methods Recombinant plasmid pQE-30-HCVe118 carrying HCV E1 gene was expressed in E.coli. coli M15 in a large amount of induction, expression; the expression of the E1 protein was isolated and purified; and then the purified protein capture HCV sera can react with HCV E1 protein IgG, IgG as a screening molecule, randomized ten Dipeptide library was panned and phage-positive clones were screened by ELISA and sequenced. As a result, the HCV E1 protein was purified with high purity, and this protein specifically reacted with sera from some hepatitis C patients. Of the 10 mimotopes obtained, 6, 2 and 2 presenting peptides have the greatest homology to the aa of 320 to 336aa, 251 to 263aa and 225 to 248 of the HCV El protein, respectively. Conclusions There are many B-cell epitopes in E1 protein, and the most probable epitope of 320-1336 in HCV E1 protein.