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目的:通过比较三种梅毒检测方法的敏感性和特异性,选择更合理的方法应用于孕产妇及新生儿梅毒患者的检测。方法:随机抽取经TPPA试验确诊的阳性血清标本33例和阴性血清标本47例,同时用上述3种方法检测,以TPPA试验作为确认试验,比较其它两种方法的灵敏度和特异度。结果:ELISA法的灵敏度及特异度分别为96.96%、95.74%;胶体金法的灵敏度及特异度分别为90.90%、93.61%;ELISA及胶体金法的试验结果与TPPA试验结果无统计学意义(x2=0.25,0;P>0.05),具有一致性。随着s/c.0.值的增高,胶体金法与ELISA法的阳性符合率也增高。结论:胶体金法的灵敏度及特异度较ELISA、TPPA方法偏低,可作为一种辅助试验。ELISA方法和TPPA方法一致性较好,可作为梅毒螺旋体抗体血清学检测的确证试验。
Objective: To compare the sensitivity and specificity of three syphilis detection methods and select more reasonable methods for the detection of syphilis in pregnant women and newborns. Methods: Thirty-three positive serum samples and 47 negative serum samples confirmed by TPPA were randomly selected and tested by the above three methods. The TPPA test was used as the confirmation test to compare the sensitivity and specificity of the other two methods. Results: The sensitivity and specificity of ELISA were 96.96% and 95.74%, respectively. The sensitivity and specificity of colloidal gold assay were 90.90% and 93.61% respectively. There was no significant difference between ELISA and colloidal gold assay and TPPA assay x2 = 0.25,0; P> 0.05), with consistency. With the increase of s / c.0., The positive coincidence rate of colloidal gold method and ELISA method also increased. Conclusion: The sensitivity and specificity of colloidal gold method is lower than that of ELISA and TPPA, which can be used as a supplementary test. ELISA method and TPPA method better consistency, as Treponema pallidum antibody serological test confirmatory test.