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为观察靶向缺氧诱导因子(HIF)1α的短发夹状小干扰RNA基因转染对骨肉瘤生长的影响,特针对HIF1α构建短发夹状siRNA真核表达载体pSilencer HIF并转染骨肉瘤细胞系(SaOS)2。将骨肉瘤细胞置于缺氧诱导培养基中培养,采用逆转录聚合酶链反应(RT PCR)和免疫印迹沉淀观察HIF1α的基因
To observe the effect of HIF-1α short hairpin RNA interference on the growth of osteosarcoma, a short hairpin siRNA eukaryotic expression vector pSilencer HIF was constructed and transfected into osteosarcoma Cell line (SaOS) 2. Osteosarcoma cells were cultured in hypoxia-inducible medium and HIF1α gene was observed by reverse transcriptase-polymerase chain reaction (RT PCR) and Western blotting