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目的:对比分析葛根芩连汤超微饮片与传统饮片葛根素溶出量,比较两种饮片药效学作用与剂量相关性,为中药复方超微饮片临床应用提供科学依据。方法:采用高效液相色谱法,测定葛根芩连汤超微饮片与传统饮片中葛根素含量;以小肠炭末推进和体外抑菌实验比较葛根芩连汤药效学作用剂量相关性。结果:葛根芩连方超微饮片葛根素溶出量增加,是传统饮片的1.37倍。药效实验结果表明,在对肠推进的最低起效浓度上,葛根芩连汤传统饮片剂量20.8g/kg,而超微饮片为1.3g/kg,是传统饮片剂量的1/16倍。在体外抑菌实验中,对于金黄色葡萄球菌、福氏痢疾和志贺痢疾三种细菌的最低抑菌浓度上,超微饮片均为传统饮片的1/2倍。结论:超微饮片细胞破壁后,化学成分溶出量较传统饮片增加,并快速溶出;同时药效学起效剂量降低,提示超微粉饮片临床用量降低,从而节约了药材资源。
OBJECTIVE: To compare and analyze the puerarin release of Gegen Qilian Decoction and traditional decoction pieces, and to compare the pharmacodynamics and dose-response of the two decoction pieces, so as to provide scientific basis for the clinical application of compound ultrafine decoction pieces. Methods: The content of puerarin in ultrafine pieces and traditional decoction pieces of Gegen Qilian Decoction was determined by high performance liquid chromatography. The pharmacodynamic effects of Gegen Qinlian decoction were compared by small intestine charcoal advancing and in vitro antibacterial experiments. RESULTS: The dissolution of puerarin from Gegenqin Lianfang Supermicro Tablets was 1.37 times that of traditional decoction pieces. The results of drug efficacy experiments showed that at the lowest concentration of bowel propulsion, Gegen Qilian Decoction traditionally consumed 20.8g/kg tablets, while ultra-microdermabrasion tablets 1.3g/kg, which was 1/16 times that of traditional drinking tablets. In the in vitro antibacterial experiment, the minimum inhibitory concentration of the three bacteria, Staphylococcus aureus, Shigella flexneri, and Shiga dysentery, was 1/2 times that of conventional decoction pieces. Conclusion: After the cell disruption of ultra-microchips, the dissolution of chemical components increased compared with the traditional decoction pieces, and the dissolution rate was rapidly released. At the same time, the dose of pharmacodynamics was reduced, suggesting that the clinical dosage of ultrafine powder tablet was reduced, thus saving the resources of medicinal materials.