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目的 探讨北京地区汉族妇女雌激素受体 (ER)基因PvuⅡ多态性与骨密度 (BMD)的关系。方法 采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)法 ,检测 179例北京地区汉族妇女ER基因PvuⅡ多态性。采用双能X线吸收仪 (DEAE)测量腰椎、近端股骨及前臂骨密度 ,并分析其相关性。结果 北京地区汉族妇女ER基因 pp、Pp、PP基因型频率分别为 0 .35 2、0 .4 6 4和 0 .184 ,绝经前与绝经后妇女无显著性差异 ,绝经后妇女腰椎骨密度为 0 .836± 0 .180 g/cm2 ,明显低于未绝经妇女的 1.0 38± 0 .14 0 g/cm2 ,绝经后妇女骨质疏松发病率为 5 4 .3%。结论 ER基因PvuⅡ基因型频率分布有种族差异。ER基因PvuⅡ基因型与骨密度无明显相关性。
Objective To investigate the relationship between Pvu Ⅱ polymorphism of estrogen receptor (ER) gene and bone mineral density (BMD) in Han women in Beijing. Methods Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) method was used to detect the polymorphism of ER gene Pvu Ⅱ in 179 Han women in Beijing. The lumbar spine, proximal femur and forearm bone mineral density were measured by dual energy X-ray absorptiometry (DEAE), and their correlations were analyzed. Results The frequencies of pp, Pp, and PP genotypes of ER genes in Beijing Han women were 0 .35 2, 0. 464 and 0.184 respectively. There was no significant difference in premenopausal and postmenopausal women. The BMD of lumbar spine in postmenopausal women was 0 .836 ± 0.80 g / cm2, which was significantly lower than that of non-menopausal women (1.0 38 ± 0.14 0 g / cm2). The incidence of osteoporosis in postmenopausal women was 54.3%. Conclusion There are racial differences in the frequency distribution of ER genotypes. ER gene Pvu Ⅱ genotype had no significant correlation with bone mineral density.