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采用人工气候箱,研究了热激萌动种子(42℃、2h)、脱落酸(ABA)(1×10-5mol/L)浸种(6 h)、2叶期叶面喷施和根部灌施,对棉苗抵御(模拟)高温干旱逆境能力的影响。结果表明,在高温干旱期间,与对照相比,所有处理都合成了差异蛋白质,但ABA浸种所合成的热激蛋白(HSP)更丰富。另外,所有处理都显著提高了棉苗超氧化物歧化酶(SOD)活性;ABA浸种、根部灌施处理和热激处理还显著提高了棉苗过氧化物酶(POD)活性;ABA叶面喷施处理和热激处理显著提高了棉苗过氧化氢酶(CAT)活性。ABA叶面喷施处理和浸种处理降低了棉苗丙二醛(MDA)含量。
The artificial climate chamber was used to study the effects of heat shock seeds (42 ℃, 2h), abscisic acid (ABA) (1 × 10-5mol / L) soaking (6h) Effect of cotton seedlings against (simulating) high temperature and drought stress ability. The results showed that during the period of high temperature and drought, compared with the control, all the treatments synthesized the differential proteins, but the heat shock proteins (HSPs) synthesized by ABA soaking were more abundant. In addition, all treatment significantly increased the superoxide dismutase (SOD) activity of cotton seedlings; ABA soaking, root irrigation and heat shock treatment also significantly increased peroxidase (POD) activity; ABA foliar spray The treatment with heat shock treatment significantly increased the catalase (CAT) activity of cotton seedlings. ABA foliar spraying and soaking treatment decreased the content of malondialdehyde (MDA) in cotton seedlings.