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感染HIV 至出现临床症状可相隔多年的体外证据为:淋巴细胞感染HIV 后长期处于低复制状态,当受抗原或促分裂原(PMA)激活后,HIV 才强烈复制并表达;血液单核细胞和组织巨噬细胞更耐受HIV的CPE,表明这两种细胞是病毒储存者。因此,建立反映HIV 感染不同阶段(急、慢性)的模型至关重要。作者建立了前单核细胞系U937急性感染HIV 后建株的UI 及T 淋巴细胞系A3、01急性感染后建株的ACH-2,用限制内切酶分析证明二株细胞均整合1～
In vitro evidence of HIV infection until many years of clinical symptoms have occurred is evidenced by long-term low replication status of lymphocytes after infection with HIV and strong replication and expression of HIV when activated by antigen or mitogen (PMA); blood mononuclear cells and Tissue macrophages are more resistant to HIV CPE, indicating that the two cells are virus reservoirs. Therefore, it is of crucial importance to establish a model that reflects the different stages of HIV infection (acute and chronic). The author established the pre-monocyte line U937 acute infection with HIV after establishment of the UI and T lymphocyte line A3,01 acute infection after the establishment of ACH-2, restriction endonuclease analysis showed that the two cells were integrated 1 ~