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基于软刻工艺发展的微血管模型为体外研究微循环系统疾病病理生理学机制提供了新思路,然而标准的软刻工艺加工的微通道呈矩形截面,不能真实地反映体内微血管管腔的圆形截面特征.本文介绍了一种简易的构建具有圆形截面特征聚二甲基硅氧烷(PDMS)微血管模型的方法.该方法以圆形截面微不锈钢丝作为模具,采用“微丝模塑抽除”软刻技术加工出具有圆形截面特征的微通道;优化微通道内细胞外基质蛋白修饰,并在修饰后的通道内进行血管内皮细胞EA.hy926灌流式培养.结果发现:纤维连接蛋白具有最佳的促进细胞黏附的效果,细胞在培养96 h后开始融合,细胞沿微通道内壁生长,保持生物活性且能承受高达2.5×10-4N/cm2的流体剪切力.本文介绍的圆形截面PDMS微血管模型加工的方法具有快速、简易的优点,可用于微循环疾病病理生理学机制的体外研究.
The microvascular model based on the development of soft etching technology provides a new idea for studying the pathophysiology of microcirculation diseases in vitro. However, the standard soft-core micromanipulation process has a rectangular cross section and can not truly reflect the circular cross-sectional features of the microvascular lumen in vivo In this paper, a simple method for constructing a PDMS microvascular model with circular cross-section is described in this paper. The method uses a micro-wire with circular cross-section as a mold, "Micro-channel with circular cross-section was processed by soft-etching technique, and the extracellular matrix protein was modified in microchannels and perfused culture in EA.hy926 cells in modified channel.The results showed that fibronectin With the best effect of promoting cell adhesion, the cells started to fuse after 96 h of culture, and the cells grew along the inner wall of the microchannel and remained bioactive and able to withstand the fluid shear force up to 2.5 × 10 -4 N / cm 2. The cross-sectional PDMS microvascular model processing method has the advantages of being quick and easy and can be used for the in vitro study of the pathophysiology of microcirculation diseases.