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目的:探讨肥大细胞(MC)在增生性瘢痕(HS)组织中的分布特征及其促纤维化作用。方法:结合MC 超微结构改变,采用甲苯胺蓝和 MC 类胰蛋白酶(MCT)免疫组织化学染色方法,观察67例面颈部烧伤后 HS患者皮肤标本和10例正常人皮肤标本,比较 MC 分布特征与病理性胶原结构形成的关系。结果:在早期瘢痕(≤6个月)组织中可见胞体肥大和 MCT 阳性颗粒丰富的 MC,其周围基质内也有 MCT 阳性物质分布,超微结构显示颗粒内容物丰富的 MC 与 FB 之间密切接触,可见胞膜融合和颗粒转移相;随着瘢痕增生和成熟,MC 密度显著下降(P<0.05～0.001),其胞膜突起和颗粒内容物明显减少,大多分布于胶原纤维间和胶原结节的边缘。结论:MC 动态分布特征和活跃的生物学行为显示其参与了 HS 胶原结构的形成。
Objective: To investigate the distribution of mast cells (MC) in hypertrophic scars (HS) and its role in promoting fibrosis. Methods: The skin specimens of HS patients and 10 normal skin samples from 67 patients with facial and neck burns were observed by immunohistochemical staining of toluidine blue and MC tryptase (MCT) in combination with ultrastructural changes of MC. MC distribution was compared Features and pathological collagen structure of the relationship. RESULTS: MCs were found in early scar tissue (≤6 months) with hypertrophy and MCT-positive granule-rich MC with positive distribution of MCT-positive substance in the surrounding matrix. The ultrastructure showed close contact between MC with abundant FB contents and FB (P <0.05-0.001), and the cell membrane and granule contents were significantly decreased, mostly distributed in collagen fibers and collagen nodules the edge of. CONCLUSION: The dynamic distribution of MC and its biological activity indicate that it participates in the formation of HS collagen.