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植物地上部分(包括叶、茎、花、果等)的表面和内部存在有大量的不同类型的细菌、丝状真菌、酵母、藻类等微生物。这种植物地上部分的生境称为叶际(phyllosphere),这些在叶际生存的微生物称为叶际微生物,它们发挥着重要的生态功能。吡虫啉是一种常用的烟碱类超高效杀虫剂,为了解它对叶际微生物产生的影响,采用磷脂脂肪酸分析(PLFA)方法评价了喷施吡虫啉杀虫剂后对桃树叶际微生物群落的影响。结果表明,桃树叶际真菌标记物18∶1ω9t磷脂脂肪酸含量最高,超过总磷脂脂肪酸含量的60%;经一遍净1号10%吡虫啉可湿性粉剂的2 000倍液处理后,叶际微生物总磷脂脂肪酸的含量、种类均有所增加,明显有别于喷水对照。PLFA主成分分析(PCA)表明,喷有吡虫啉农药的叶片样品叶际微生物群落结构与喷水样品、空白对照样品差异明显,并且增加吡虫啉处理次数会增强其对叶际微生物群落影响的显著性。
There are a large number of different types of bacteria, filamentous fungi, yeasts, algae and other microorganisms on the surface and inside of plant parts (including leaves, stems, flowers, fruits, etc.) The habitats of the aerial parts of this plant are called phyllospheres. These microorganisms that live on the leafy fronds are called inter-leaf microorganisms and play an important ecological role. Imidacloprid is a commonly used nicotine super-efficient insecticide. In order to know its influence on the inter-leaf microorganisms, phospholipid fatty acid analysis (PLFA) was used to evaluate the effects of spraying imidacloprid pesticides on the microbial community influences. The results showed that the content of 18:1ω9t phospholipid fatty acid in peach leaf seminal fungus marker was the highest, which exceeded 60% of the total phospholipid fatty acid content. After treated with 10% imidacloprid WP 1 000 times, Fatty acid content, types have increased, obviously different from the water control. The results of PLFA principal component analysis (PCA) showed that the leaf community with imidacloprid pesticide sprayed leaves showed significant differences in the leaf community structure and water spray samples and blank control samples. Increasing the number of imidacloprid treatments increased the significant impact on the leaf microbial community.