论文部分内容阅读
连续对转玉米磷酸烯醇式丙酮酸羧化酶 (PEPC)基因水稻植株PEPC活性进行测定,选取PEPC为1 200μmol/mg·h以上的稳定的转PEPC基因水稻H7 代种质(命名为HPTER)为父本,以水稻品种 (系 )9516为母本,通过杂交获得F1 杂种,对其进行花药培养,获得 189个再生植株,其中二倍体植株占总株数的 65 6%。测定了71个株系的PEPC活性,鉴定出 3个株系:H137、H45和H119,其光合效率较对照 9516增加 18 9% ~25 1%,单株产量较对照增加 7 1% ~29 8%。对H45进行剂量为150Gy、250Gy、300Gy和350Gy的γ射线辐照,选育出生育期与 9516相近,重穗、大粒的株系JAAS45。本研究结果证明,通过定向选育的高表达转PEPC基因水稻种质HPTER,采用杂交、花药培养及γ射线辐照等方法,可以将玉米PEPC基因快速转移到普通水稻品种中,培育出高光合效率和高产的水稻品系。
The PEPC activity of PEPC transgenic rice plants was continuously measured. The PEPC transgenic rice H7 lines (named as HPTER) with a PEPC of more than 1200 μmol / mg · h were selected, As the male parent, F1 hybrids were obtained from the hybrid rice 9516 using the rice variety (line 9516) as the female parent, and 189 hybrid plants were obtained by anther culture. Among them, diploid plants accounted for 65.6% of the total number of plants. The PEPC activities of 71 lines were determined and three lines were identified: H137, H45 and H119, their photosynthetic efficiency increased by 18 9% -25 1% compared with that of the control 9516, and the yield per plant increased by 7 1% -29 8 %. H45 was irradiated with γ-rays of 150Gy, 250Gy, 300Gy and 350Gy, and strain JAAS45 with a similar growth period and 9516 spikelet size was selected. The results of this study demonstrated that the PEPC gene of maize can be rapidly transferred to common rice varieties through directional selection and high-expression transformation of PTERC rice germplasm HPTER, using hybridization, anther culture and γ-ray irradiation, Efficiency and high yield of rice lines.