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为进一步探讨慢性粒细胞白血病 (慢粒 )的分期、分型和治疗 ,对 6 0例患者的临床资料进行了分析 ,并用R显带技术检查了 49例患者的染色体核型 ,用逆转录 -聚合酶链反应法检查了 13例bcr/abl融合基因。结果发现慢粒急变期Hb和BPC明显低于慢性期 ;5例为Ph(- ) ,44例为Ph(+) ;6 6 .7%的慢粒急变患者有额外染色体异常 ;5例行异基因骨髓移植术后转为正常核型 ;8例Ph(+)患者bcr/abl基因亦阳性 ,5例Ph(- )患者有 2例阳性 ;10例慢粒急变者经治疗达完全缓解。这些结果提示染色体检查及bcr/abl融合基因检测有助于慢粒的诊断、鉴别诊断、预测急变和判断疗效。异基因骨髓移植是根治慢粒的有效方法。
In order to further investigate the staging, typing and treatment of chronic myeloid leukemia (CML), the clinical data of 60 patients were analyzed and the chromosomal karyotypes of 49 patients were examined by R - banding technique. 13 cases of bcr / abl fusion gene were examined by polymerase chain reaction. The results showed that Hb and BPC were significantly lower than those in the chronic phase of CML; Ph (-) in 5 cases and Ph (+) in 44 cases; extrachromosomal abnormalities were found in 66.7% of CML patients; Bcr / abl gene was also positive in 8 patients with Ph (+), 2 patients were positive in 5 patients with Ph (-), and 10 patients with CML completely remission after treatment. These results suggest that chromosomal examination and bcr / abl fusion gene detection contribute to the diagnosis of CML, differential diagnosis, prediction of acute changes and to determine the efficacy. Allogeneic bone marrow transplantation is an effective method to cure CML.