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目的观察淫羊藿苷对体外培养小鼠破骨细胞β-肌动蛋白、抗酒石酸酸性磷酸酶(TRAP)、核因子-κB受体活化因子(RANK)mRNA表达的影响。方法用浓度分别为25、30μg/L、1×10~(-8)mol/L的巨噬细胞集落刺激因子(M-CSF)、核因子-κB受体活化因子配体(RANKL)、1,25(OH)_2VitD3体外诱导培养小鼠骨髓源性破骨细胞,分别加入0、1×10~(-5)mol/L的淫羊藿苷,培养48 h后,抽提总RNA,采用半定量逆转录-聚合酶链反应(RT-PCR)方法检测破骨细胞中β肌动蛋白、TRAP、RANK mRNA表达的变化。结果与空白对照组比较,1×10~(-5)mol/L的淫羊藿苷可明显下调β-肌动蛋白、RANK mRNA的表达(P值均<0.05),但对TRAP mRNA的表达无明显影响(P>0.05)。结论淫羊藿可以通过下调β-肌动蛋白、RANK基因表达,从而抑制破骨细胞的分化形成及骨吸收。
Objective To observe the effects of icariin on the expression of β-actin, TRAP, and RANK mRNA in cultured mouse osteoclasts. Methods Macrophage colony stimulating factor (M-CSF), nuclear factor-kappa B receptor activating factor ligand (RANKL), 1 were used at concentrations of 25, 30 μg/L and 1×10 -8 mol/L, respectively. In vitro, mouse bone marrow-derived osteoclasts were induced with 25(OH)_2VitD3 and 0 and 1×10 -5 mol/L of icariin was added. After 48 hours of culture, total RNA was extracted. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the changes of β-actin, TRAP and RANK mRNA expression in osteoclasts. Results Compared with the control group, 1×10 -5 mol/L icariin significantly down-regulated the expression of β-actin and RANK mRNA (P<0.05), but the expression of TRAP mRNA No significant effect (P>0.05). Conclusion Epimedium can inhibit osteoclast differentiation and bone resorption by down-regulating β-actin and RANK gene expression.