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骨型碱性磷酸酶(ALP)和骨钙素由骨母细胞合成被用作骨质更新的生化标志。本文以一组有骨质疏松危险性的健康老年妇女为实验对象,比较了测定骨型ALP的两种方法:即新近发展起来的双位点免疫放射测定法(two-site IRMA)和麦胚凝集素(WGA)沉淀法,并对骨型ALP与总ALP活性和骨钙素作了相关性分析。以P-硝基磷酸苯酯为底物,于30℃用Hitachi737分析仪测定ALP活性。骨钙索的分析采用RIA。结果WGA溶液的稳定性较差(室温3天)。与NaCl(9g/L).PBS pH7.4,50mmol/L Tris缓冲液pH7.4,或者50g/L小牛(或人)血清白蛋白相比,用双蒸水稀释高浓度的样品所得实验结果比较可靠。WGA沉淀分析法的线性范围在300U/L之内。WGA沉淀分析法与IRMA法分析骨型ALP的误差类似。用两种方法分析101名70岁以上妇女
Bone alkaline phosphatase (ALP) and osteocalcin are synthesized by osteoblasts and are used as biochemical markers of bone turnover. In this study, a group of healthy elderly women at risk of osteoporosis were included in this study. Two methods for the determination of bone type ALP were compared: the newly developed two-site IRMA and wheat germ Lectin (WGA) precipitation method, and ALP and bone ALP activity and osteocalcin were analyzed. ALP activity was measured on a Hitachi 737 analyzer using P-nitrophenyl phosphate as a substrate at 30 ° C. Osteocalcin analysis using RIA. Results The stability of WGA solution was poor (3 days at room temperature). Compared with NaCl (9 g / L). PBS pH 7.4, 50 mmol / L Tris buffer pH 7.4 or 50 g / L calf (or human) serum albumin, The result is more reliable. The linear range of the WGA precipitation assay is within 300 U / L. WGA precipitation analysis and IRMA analysis of bone ALP similar error. Two methods were used to analyze 101 women over the age of 70