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目的:研究哺乳期母鼠接触磺胺间甲氧嘧啶(SMM)对其仔鼠早期生长发育的影响。方法:24只孕鼠分娩后均分为SMM对照组、低、中、高剂量组,即0、10、50、200 mg/kg,仔鼠出生后第1天开始灌胃暴露直至21天,于第1、7、14、21天称量鼠的体重,第22天断头处死取血清,放射免疫法测量三碘甲腺原氨酸(T3)、甲状腺激素(T4)、胰岛素(INS)、生长激素(GH),酶联免疫法测胰岛素样生长因子1(IGF-1)。结果:各剂量组在出生后第1、7、14和21天仔鼠体重差异均无统计学意义(P>0.05)。与对照组相比,高剂量组第3周体重增长百分比(61.5±6.4)%降低,差异有统计学意义(P<0.05);高剂量组的INS水平(32.84±8.28)μIU/ml升高,差异有统计学意义(P<0.05)。T4、T3、GH和IGF-1各剂量组之间差异无统计学意义(P>0.05)。结论:哺乳期母鼠接触SMM影响了子代小鼠早期的生长发育。
OBJECTIVE: To study the effects of exposure to sulfamonomethoxine (SMM) on the early growth and development of offspring in nursing mothers. Methods: Twenty-four pregnant rats were divided into three groups: SMM control group, low, medium and high dose groups, ie 0, 10, 50 and 200 mg / kg. The body weight of the mice was weighed on the 1st, 7th, 14th and 21st days. The serum was decapitated on the 22nd day, and the levels of T3, T4, INS were measured by radioimmunoassay. , Growth hormone (GH) and insulin-like growth factor 1 (IGF-1) by enzyme-linked immunosorbent assay. Results: There was no significant difference in the body weight of pups on the 1st, 7th, 14th and 21st day after birth in each dose group (P> 0.05). Compared with the control group, the percentage of body weight gain in the high-dose group decreased by 61.5 ± 6.4% at the third week (P <0.05), but the INS level increased by 32.84 ± 8.28 μIU / ml in the high-dose group , The difference was statistically significant (P <0.05). T4, T3, GH and IGF-1 of each dose group showed no significant difference (P> 0.05). CONCLUSIONS: Lactating maternal exposure to SMM affected the early growth of offspring mice.