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采用农杆菌介导法将外源三价抗虫基因Bt- Cp TI- GN A导入彩色棉中,经分子检测已整合到受体植株体内,并已得到表达,目前已得到纯化株行.外源标记基因N PTII检测在T0 代就有分离现象,各代检测淘汰3～4次才能基本清除分离株或非转化株.抗虫性检测表明转三价抗虫基因彩色棉对棉铃虫具有较高抗性,而对棉蚜则作用不大.
Agrobacterium-mediated transformation of the exogenous bivalent insect-resistant gene Bt-Cp TI-GN A into the color cotton, the molecular detection has been integrated into the recipient plant and has been expressed, has been purified strains. The source marker gene N PTII detection in the T0 generation there is a separation phenomenon, the detection of each generation to eliminate 3 or 4 times to basically eliminate the isolate or non-transformed strains. Insect resistance test showed that the three transgenic cotton insect resistant cotton bollworm High resistance, but little effect on cotton aphids.