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目的 探讨人胃癌细胞株对不同化疗药物、α 干扰素及其相互配伍的敏感性。方法 向体外培养的人胃癌细胞 (MGC80 - 3)中加入不同配伍及不同浓度的化疗药物和α 干扰素 ,作用一定时间后 ,采用MTT法检测癌细胞对不同配伍药物的敏感性。结果 联合用药较单一用药的相对抑制率明显增高 ,尤其以氟尿嘧啶、丝裂霉素和表阿霉素 3药联合作用的抑制率最高 ;随药物剂量的增加 ,各组的抑制率有所增高 ,α 干扰素与化疗药物合用时 ,有协同抑制作用 ,尤其在先用α 干扰素 2 4小时后 ,再加用化疗药物 ,抑制率明显增高。结论 联合用药化疗疗效优于单一用药 ,加用α 干扰素具有协同抑制作用 ,尤以先用α 干扰素后 ,再联合用化疗药 ,疗效最佳。
Objective To investigate the sensitivity of human gastric cancer cell lines to different chemotherapeutic drugs, interferon-α and their mutual compatibility. Methods The human gastric cancer cells (MGC80 - 3) cultured in vitro were treated with different concentrations and concentrations of chemotherapy drugs and interferon - α. After a certain period of time, MTT assay was used to detect the sensitivity of cancer cells to different compatible drugs. Results The relative inhibition rate of combination therapy was higher than that of single therapy, especially the combination of fluorouracil, mitomycin and epirubicin was the highest. With the increase of drug dose, the inhibition rate of each group was increased, Alpha interferon combined with chemotherapy drugs, synergistic inhibition, especially in the first with interferon alpha 2 4 hours, coupled with chemotherapy drugs, the inhibition rate was significantly higher. Conclusions Combination chemotherapy is more effective than single therapy. Adding IFN-α has a synergistic inhibitory effect, especially after using interferon-alpha first, combined with chemotherapeutic drugs.