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Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.
Zucchini yellow mosaic virus (ZYMV) was detected by RT-PCR from pumpkin (Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng, Shandong Province. 3’-term 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence, 840 bp of cp gene and 211 bp of 3’-untranslated region of the isolate ZYMV-Liaocheng was determined. The cp gene of ZYMV-Liaocheng shared identities of 81.4% -98.8% and 89.4% -99.5% at nucleotide and amino acid levels, respectively, with other ZYMV sequences available in the GenBank. Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes. ZYMV-Liaocheng belonged to genotype I, which contained isolates from Asia, Europe and America. Genotypes III and V were unique and contained only isolates from East Asia. the isolates from East Asia had the highest variability.