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目的 探讨胶质瘤p2 7Kip1,bcl 2和PCNA蛋白表达与肿瘤恶性程度、细胞增殖活性、凋亡程度的关系。方法 采用免疫组化染色S P法检测 66例不同级别的胶质瘤 p2 7Kip1,bcl 2和PCNA蛋白的表达。结果 在 66例胶质瘤中 ,p2 7Kip1表达 18例(2 7% ) ,bcl 2表达 2 0例 (3 0 % ) ,PCNA表达 5 1例 (77% )。p2 7Kip1蛋白表达率随着胶质瘤级别升高而减少 ;bcl 2蛋白表达率随肿瘤级别升高而相应增加 ;PCNA表达随胶质瘤级别升高阳性反应强度增加 ;但Ⅰ、Ⅱ与Ⅲ级和Ⅳ级组间无显著性差异。结论 p2 7Kip1蛋白表达的缺失可能与胶质瘤的发生有关 ;bcl 2基因可能间接抑制细胞凋亡而与胶质瘤的分型、细胞的增殖活性以及潜在的临床行为无直接关系 ;PCNA的表达与星形胶质细胞瘤的恶性行为有关
Objective To investigate the relationship between the expression of p27Kip1, bcl2 and PCNA in glioma and the degree of malignancy, cell proliferation and apoptosis. Methods Immunohistochemical SP method was used to detect the expression of p27Kip1, bcl2 and PCNA in 66 cases of gliomas. Results In 66 gliomas, p27Kip1 expression was found in 18 cases (27%), bcl 2 expression in 20 cases (30%), and PCNA expression in 51 cases (77%). The expression of p2 7Kip1 protein decreased with the increase of glioma grade. The expression of bcl2 protein increased with the increase of tumor grade. The expression of PCNA increased with the increase of glioma grade. However, the expression of Ⅰ, Ⅱ and Ⅲ There was no significant difference between grade and grade IV. Conclusion The loss of p2 7Kip1 protein may be related to the pathogenesis of gliomas. The bcl 2 gene may indirectly inhibit the apoptosis of glioma cells, and may be directly related to the type of glioma, cell proliferation activity and potential clinical behavior. The expression of PCNA And astrocytoma malignant behavior