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通过观察小鼠热应激模型的生长指标、免疫器官指数、血清IL-2及激素的动态水平,探讨富硒女贞子抗热应激的调节作用。将108只ICR小鼠随机分成6组(每组设3个重复),分别为常温对照组(C)、热应激组(HS)、普通女贞子组(1.5 g/kg,CLL)和富硒女贞子组(剂量分别为0.75 g/kg、1.5 g/kg、3.0 g/kg,分别表示为HSLL、MSLL、LSLL)。除C组外,其他各组于T:(40±1)℃,RH:40%~50%人工气候室中,11:00-13:00热应激2 h,应激期间不间断供水和药液(自由饮用);其余时间置于白天T:(35±4)℃,RH:45%~50%环境下,夜晚T:(28±5)℃,RH:30%~40%环境下,连续高温应激7 d。结果表明,在高温应激条件下,LSLL组的增重率(WG)和生长率(SGR)分别显著和极显著高于HS组(P<0.05,P<0.01);CLL组、MSLL组、LSLL组免疫器官指数均显著高于HS组(P<0.05);热应激前期(1~3 d),HS组血清中促肾上腺皮质激素(ACTH)浓度显著增高(P<0.01),CLL、MSLL和LSLL组均无显著差异(P>0.05);HS组的皮质醇(CORT)浓度较C组显著升高(P<0.05),女贞子各组均有降低,其中MSLL组与C组无显著差异(P>0.05);HS组血清中的T3、T4浓度总体上呈现下降的趋势,但是CLL、LSLL组的T3、T4浓度均显著高于HS组(P<0.01);IL-2方面,在热应激前期(1~3 d),富硒女贞子HSLL组、LSLL组的IL-2显著高于HS组和C组(P<0.05,P<0.01)。结果提示,富硒女贞子和女贞子对热应激造成小鼠生长不良及免疫器官损伤均具有明显的缓解作用,并能够增强热应激小鼠免疫调节功能,通过机体的内分泌-免疫调节网络系统,改善机体的内环境稳态,发挥提高动物耐热能力的作用,且以低剂量(0.75 g/kg)富硒女贞子组作用最佳。
By observing the growth index, immune organ index, serum IL-2 level and the dynamic level of hormones in heat-stress mouse models, we explored the regulatory effect of Se-enriched Ligustrum lucidum on heat stress. A total of 108 ICR mice were randomly divided into 6 groups (3 replicates per group), which were normal temperature control group (C), heat stress group (HS), normal Ligustrum lucidum group (1.5 g / kg, Selenium-enriched Ligustrum lucidum subgroups (doses of 0.75 g / kg, 1.5 g / kg, 3.0 g / kg, respectively, indicated as HSLL, MSLL, LSLL). In addition to group C, the other groups in the T: (40 ± 1) ℃, RH: 40% to 50% of the artificial climate chamber, 11: 00-13: 00 heat stress 2 h, uninterrupted water supply during stress and (35 ± 4) ℃, RH: 45% ~ 50%, night T: (28 ± 5) ℃, RH: 30% ~ 40% , Continuous high-temperature stress 7 d. The results showed that under high temperature stress, the weight gain rate (WG) and growth rate (SGR) of LSLL group were significantly and extremely significantly higher than those of HS group (P <0.05, P <0.01) The immune organ index of LSLL group was significantly higher than that of HS group (P <0.05). The serum adrenocorticotrophic hormone (ACTH) concentrations in HS group were significantly increased (P <0.01) in the early stage of heat stress (1-3 days) There was no significant difference between MSLL group and LSLL group (P> 0.05). Cortisol (CORT) concentration in HS group was significantly higher than that in C group (P <0.05) (P> 0.05). The concentrations of T3 and T4 in serum of HS group tended to decrease, but the concentrations of T3 and T4 in CLL and LSLL group were significantly higher than those in HS group (P <0.01) In the early stage of heat stress (1 ~ 3 d), IL-2 of LSLL group and LSLL group were significantly higher than HS group and C group (P <0.05, P <0.01). The results suggest that selenium-enriched Ligustrum lucidum and Ligustrum lucidum have a significant relieving effect on the growth of mice and the damage of immune organs induced by heat stress, and can enhance the immunomodulatory function of heat-stressed mice through endocrine-immune Regulate the network system, improve the homeostasis of the body, and play an important role in improving the heat tolerance of animals, and the effect is best at the low dose (0.75 g / kg) selenium-rich Ligustrum lucidum subgroup.