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目的:探索结直肠癌患者术前术后肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、一氧化氮(nitric oxide,NO)、白细胞介素-1β(Interleukin-1Beta,IL-1β)、白细胞介素-6(interleukin-6,IL-6)表达水平的变化及其临床意义。方法:对本院75例结直肠癌患者分别采用硝酸还原法及双抗夹心ELISA法检测手术前后NO及TNF-α、IL-1β、IL-6表达水平的变化,同时将其与43例健康体检者相比较。结果:结直肠癌患者手术前血清中TNF-α、IL-1β、IL-6浓度(19.45±4.09,3.91±0.25,15.93±1.00)明显高于正常对照组(P<0.05),而NO浓度(27.5±5.5)明显低于正常对照组(P<0.05),手术治疗后7天,TNF-α、IL-1β、IL-6血清含量(12.31±3.89,1.41±0.33,9.30±2.11)明显降低(P<0.05),而NO含量恢复正常水平。结论:血清中TNF-α、NO、IL-1β、IL-6浓度与结直肠癌密切相关,对TNF-α、NO、IL-1β、IL-6浓度进行联合诊断可作为结直肠癌早期诊断的参考指标。
Objective: To explore the correlation between tumor necrosis factor-α (TNF-α), nitric oxide (NO), interleukin-1β (IL- 1β, IL-6 and their clinical significance. Methods: The changes of NO and TNF-α, IL-1β and IL-6 levels in 75 patients with colorectal cancer before and after operation were detected by nitrate reduction and double antibody sandwich ELISA respectively. Meanwhile, they were compared with 43 healthy controls Physical examination compared. Results: The serum levels of TNF-α, IL-1β and IL-6 in patients with colorectal cancer before surgery were significantly higher than those in normal controls (19.45 ± 4.09,3.91 ± 0.25,15.93 ± 1.00, P <0.05) (27.5 ± 5.5) was significantly lower than that of the normal control group (P <0.05). Serum TNF-α, IL-1β and IL-6 levels were significantly higher at 7 days after operation (12.31 ± 3.89, 1.41 ± 0.33, 9.30 ± 2.11) Decreased (P <0.05), while the content of NO returned to normal level. CONCLUSION: Serum levels of TNF-α, NO, IL-1β and IL-6 are closely related to colorectal cancer. Combined diagnosis of TNF-α, NO, IL-1β and IL-6 may be used as an early diagnosis of colorectal cancer The reference index.