Objectives To assess the relationship between peak oxygen consumption(PVO2) and the ambulation distance in six-minute walk test (6MWT)among the healthy subjects. Methods The 51 healthy subjects were recruited for the six-minute walk test. Data of pulmonary gas exchange breath by breath, such as VO2, VCO2 were real-time measured with wireless remote sensing K4B2, so to study the relationship between peak oxygen uptake and the ambulation distance. Results It was noticed that there was a positive linear correlation between the ambulation distance and PVO2 (r=0.619, P<0.001) in six-minute walk test. The regression equation was set up (VO2/kg=0.05D-6.331, P<0.001). PVO2>PVCO2,R<1 were found,which suggested that 6MWT was a test below anaerobic threshold. Conclusions There was a closely positive linear correlation between the ambulation distance and PVO2, which is safety, convenient and valuable for the evaluation of cardiopulmonary function and the treatment of cardiopulmonary rehabilitation. Objectives To assess the relationship between peak oxygen consumption (PVO2) and the ambulation distance in six-minute walk test (6MWT) among the healthy subjects. Methods The 51 healthy subjects were recruited for the six-minute walk test. Data of pulmonary gas exchange breath by breath, such as VO2, VCO2 were real-time measured with wireless remote sensing K4B2, so to study the relationship between peak oxygen uptake and ambulation distance. Results It was noticed that there was a positive linear correlation between the ambulation distance and The regression equation was set up (VO2 / kg = 0.05D-6.331, P <0.001). PVO2> PVCO2, R <1 were found, which suggested that 6MWT was a test below anaerobic threshold. Conclusions There was a closely-positive linear correlation between the ambulation distance and PVO2, which is safety, convenient and valuable for the evaluation of cardiopulmonary function and the treatment of cardiopulmonary rehabilitation .