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[目的]为提高解淀粉芽孢杆菌Bs2-4防治烟草青枯病效果,筛选出与Bs2-4菌株复配药剂及比例。[方法]采用抑菌圈法测定7种杀菌剂和Bs2-4菌对烟草青枯病菌的毒力,计算EC50值。平板菌落计数法测定杀菌剂与Bs2-4菌生物相容性,Horsfall法确定复配方案。[结果]硫酸链霉素抑制中质量浓度最低,为170.01 mg/L,且与Bs2-4菌有较好生物相容性。170.01 mg/L硫酸链霉素与3.3×107 cfu/m L Bs2-4菌体积比为4∶6时,IR值为1.232。复配剂对烟草青枯病菌生长抑制率达68.59%,烟草青枯病防效达65.85%,显著高于单剂防效。[结论]硫酸链霉素可与解淀粉芽孢杆菌Bs2-4菌联用防治青枯病,混剂施用减少一半化学农药施用量。
[Objective] The research aimed to improve the effect of Bacillus amyloliquefaciens Bs2-4 against tobacco bacterial wilt and screen out the compound with Bs2-4 strain and its proportion. [Method] The virulence of 7 fungicides and Bs2-4 strains against R. solanacearum was determined by inhibition zone method and the EC50 value was calculated. The biocompatibility of bactericide and Bs2-4 was determined by plate colony counting method, and the compounding scheme was determined by Horsfall method. [Result] Streptomycin sulfate had the lowest concentration of streptomycin, which was 170.01 mg / L and had good biocompatibility with Bs2-4. The IR value was 1.232 when the volume ratio of streptomycin sulfate to 3.3 × 107 cfu / mL Bs2-4 was 4: 6. The inhibitory rate of the compound against tobacco bacterial wilt was 68.59%, and the control effect of tobacco bacterial wilt reached 65.85%, which was significantly higher than that of the control. [Conclusion] streptomycin sulfate could be used in combination with Bacillus amyloliquefaciens Bs2-4 to control bacterial wilt. The mixture of pesticides was reduced by half of chemical pesticides.