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目的 ]探讨母亲微粒体环氧化酶 (EPHX1)与谷胱甘肽 S转移酶 (GSTM1,GSTT1)的遗传多态性对新生儿出生体重的影响。 [方法 ]采用回顾性流行病学调查方法 ,使用统一调查表 ,由经过培训的调查员在北京燕山某医院对入院分娩的 6 30个母亲婴儿对进行调查。 [结果 ]单因素分析发现 :EPHX1和 GSTT1、GSTM1基因多态对新生儿出生体重的影响无显著意义。经母亲年龄、文化程度、生育史、芳香烃溶剂暴露、在家被动吸烟、孕前体重指数、新生儿孕周与性别因素调整后 ,也未见有显著意义。然而对基因 EPHX1,GSTT1,和 GSTM1综合分析时发现 :EPHX1杂合子 /突变基因型合并 GSTT1缺失基因型的母亲其新生儿出生体重显著低于 EPHX1纯合子 /野生型合并GSTT1存在基因型 ;EPHX1杂合子 /突变基因型合并 GSTM1缺失基因型的母亲其新生儿体重显著低于 EPHX1纯合子 /野生基因型合并 GSTM1存在基因型。 [结论 ]基因 EPHX1与基因 GSTT1,GSTM1之间对新生儿出生体重的影响存在交互作用 ,这为阐明不良生殖结局的病因提供了新的研究模式
Objective] To investigate the effects of genetic polymorphisms of maternal microsomal cyclooxygenase (EPHX1) and glutathione S-transferase (GSTM1, GSTT1) on newborn birth weight. [Methods] A retrospective epidemiological survey method was used to survey 630 mothers who were hospitalized and delivered by a trained investigator at a hospital in Yanshan, Beijing, using a unified questionnaire. [Results] Univariate analysis showed that the polymorphisms of EPHX1, GSTT1 and GSTM1 had no significant effect on newborn birth weight. There was no significant difference after adjustment for maternal age, education level, birth history, exposure to aromatic solvents, passive smoking at home, body mass index before pregnancy, neonatal gestational age and gender. However, a comprehensive analysis of genes EPHX1, GSTT1 and GSTM1 revealed that mothers with EPHX1 heterozygote / mutant genotypes with GSTT1 deletion genotype had significantly lower birth weights than those with EPHX1 homozygous / wild-type GSTT1 genotypes; EPHX1 heterozygous Homozygous / mutant genotype males with GSTM1-null genotype had significantly lower birth weights than EPHX1 homozygous / wild-type genotypes with GSTM1-present genotype. [Conclusion] There is an interaction between gene EPHX1 and GSTT1 and GSTM1 on the birth weight of neonate, which provides a new research mode for elucidating the etiopathogenisis of adverse reproductive outcomes