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目的:探讨生长抑制因子1(ING1)基因表达及其与胃癌临床病理特征的关系。方法:荧光定量PCR技术和免疫印迹技术检测70例胃腺癌组织及癌旁对照胃黏膜组织中ING1基因的表达水平,并分析其与胃癌患者临床特征的关系。结果:70例癌组织中有57例(81.43%)ING1基因mRNA表达明显下调,其中4例组织为ING1表达缺失,与癌旁正常胃黏膜比较差异有统计学意义,P<0.01。ING1基因表达与胃癌患者的性别无明显相关性,与胃癌的分化程度、肿瘤累及深度以及淋巴结转移有关。结论:ING1在人胃癌细胞中表达下调,其表达水平与分化程度、肿瘤累及深度以及淋巴结转移有关。
Objective: To investigate the relationship between the expression of growth inhibitory factor 1 (ING1) gene and clinicopathological features of gastric cancer. Methods: The expression of ING1 gene in 70 gastric adenocarcinoma tissues and adjacent non-cancerous gastric mucosa tissues was detected by fluorescence quantitative PCR and Western blotting. The relationship between ING1 gene expression and clinical features of gastric cancer was analyzed. Results: The mRNA expression of ING1 was significantly down-regulated in 57 of 70 (81.43%) cancerous tissues, of which 4 were negative for ING1 expression, which was significantly different from normal gastric mucosa (P <0.01). There was no significant correlation between the expression of ING1 gene and the gender of gastric cancer patients, which was related to the differentiation of gastric cancer, depth of tumor invasion and lymph node metastasis. CONCLUSION: The expression of ING1 is down-regulated in human gastric cancer cells. The expression level of ING1 is related to the degree of differentiation, depth of tumor invasion and lymph node metastasis.