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目的 测定培养状态下间皮细胞的抗凝与纤溶作用的强度并与内皮细胞、成纤维细胞作比较 ,为血管假体内腔铺被细胞的选择提供依据。方法 取SD大鼠大网膜、主动脉、皮下结缔组织作间皮、内皮及成纤维细胞的培养。取 3类细胞第 3代融合后 4 8h的无血清培养液 ,放射免疫法测定 6 酮 PGF1α(前列环素的代谢产物 )的含量 ,发色底物法测定纤溶酶原激活物活性。结果 间皮细胞培养液中6 酮 PGF1α浓度显著高于成纤维细胞及内皮细胞 ,纤溶酶原激活物活性也高于成纤维细胞 ,但与内皮细胞相比差异无显著性。结论 培养状态下的间皮细胞具有与内皮细胞相似的功能特点 ,可能成为血管假体内腔铺被层的理想材料。
Objective To determine the intensity of anticoagulant and fibrinolytic effect of mesothelial cells cultured in vitro and to compare with endothelial cells and fibroblasts to provide a basis for the selection of endothelium-coated cells in vascular prostheses. Methods The omentum, endothelium and fibroblasts of the omentum, aorta and subcutaneous connective tissue of SD rats were cultured. Serum-free medium of 48h after the 3rd generation of 3 types of cells fusion was collected. The content of 6-keto PGF1α (the metabolite of prostacyclin) was determined by radioimmunoassay. The activity of plasminogen activator was determined by chromogenic substrate method. Results The concentration of 6-keto-PGF1α in mesothelial cells was significantly higher than that in fibroblasts and endothelial cells. The activity of plasminogen activator was also higher than that of fibroblasts, but no significant difference compared with endothelial cells. Conclusion The cultured mesothelial cells have the similar functional characteristics as endothelial cells, which may be the ideal material for vascular prosthesis lining.