论文部分内容阅读
花生工业界认为收获前花生黄曲霉毒素的污染是全世界花生工业界面临的最严峻的挑战。干旱胁迫是加重花生黄曲霉真菌侵染和毒素污染最重要的环境因素。选育花生抗性品种将使美国花生工业处于优势地位。在这一研究报告中 ,我们鉴定出了一个新的类PLD基因 ,它编码磷脂酶D。在植物体中 ,这个酶是负责干旱诱导降解细胞膜磷脂的主要酶。克隆的PLD1片段有 1 0 69个核甘酸对长。推导的氨基酸序列与已知的PLD基因有很高的同一性 ,包括相似的保守序列特征 ,比如两个HXKXXXXD基元。对花生PLD基因特性需要从遗传和生理上作进一步研究 ,以便更好地理解这个基因的功能及其与花生耐干旱性的关系
Peanut industry believes that the pollution of aflatoxin in peanut before harvest is the most serious challenge that the peanut industry in the world faces. Drought stress is the most important environmental factor that aggravates fungal infection of Aspergillus flavus and toxin contamination. Breeding of resistant varieties of peanuts will enable the U.S. peanut industry to take a dominant position. In this study, we identified a novel PLD-like gene that encodes phospholipase D. In plants, this enzyme is the main enzyme responsible for drought-induced degradation of cell membrane phospholipids. The cloned PLD1 fragment has 1 0 69 pairs of nucleotides long. The deduced amino acid sequence shares a high degree of identity with known PLD genes, including similar conserved sequence features, such as two HXKXXXXD motifs. The peanut PLD gene profile needs to be further studied both genetically and physiologically in order to better understand the function of this gene and its relationship to drought resistance in peanut