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目的 :观察褪黑素 (MT)对吗啡戒断大鼠不同脑区cAMP和cGMP含量的影响。方法 :以剂量递增法连续皮下注射吗啡建立吗啡依赖模型 ,采用放射免疫学方法测定脑内cAMP和cGMP的含量。结果 :(1)MT对大鼠吗啡戒断症状具有明显的抑制作用 ;(2 )与对照组比较 ,吗啡依赖大鼠的纹状体、间脑、中脑、脑桥和海马内cAMP含量显著增高 (P <0 0 5 ,P <0 0 1) ,cGMP含量显著下降 (P <0 0 5 ,P <0 0 1)。与吗啡依赖组比较 ,催促戒断大鼠海马和纹状体内cAMP的含量显著升高 (P <0 0 5 ) ,而cGMP含量显著下降 (P <0 0 5 ,P <0 0 1) ,其他部位则无明显变化 ;(3)褪黑素急性治疗可使吗啡戒断大鼠纹状体、间脑、中脑、脑桥和海马内cAMP含量明显下降 (P <0 0 5 ,P <0 0 1) ,cGMP含量明显增高 (P <0 0 5 ,P <0 0 1)。结论 :MT可显著抑制大鼠吗啡戒断反应 ,并与调节中枢cAMP和cGMP含量有关。
Objective: To observe the effect of melatonin (MT) on cAMP and cGMP in different brain regions of morphine withdrawal rats. Methods: Morphine-dependent model was established by continuous subcutaneous injection of morphine by dose escalation and cAMP and cGMP levels were measured by radioimmunoassay. Results: (1) MT significantly inhibited morphine withdrawal symptoms in rats; (2) Compared with the control group, cAMP content in striatum, diencephalon, midbrain, pons and hippocampus of morphine dependent rats was significantly increased (P <0.05, P <0.01), and the cGMP content decreased significantly (P <0.05, P <0.01). Compared with the morphine dependent group, the content of cAMP in hippocampus and striatum was significantly increased (P <0 05), while the content of cGMP was significantly decreased (P 0 05, P 0 01) in other rats (3) The acute treatment of melatonin could significantly decrease the cAMP content in striatum, diencephalon, midbrain, pons and hippocampus of morphine withdrawal rats (P <0.05, P <0 0 1), cGMP content was significantly increased (P <0 05, P <0 01). CONCLUSION: MT can significantly inhibit the morphine withdrawal response in rats and is related to the regulation of cAMP and cGMP contents in the central nervous system.