论文部分内容阅读
目的 为胎鼠脊髓前角神经元的分离培养提供可靠而稳定的方法。方法 以 6 .8%Metri zamide形成的密度梯度体系离心与抑制有丝分裂相结合进行胎鼠脊髓前角神经元原代分离培养 ,并用NF 2 0 0免疫组化染色后图象分析了解神经元的生长规律。结果 所获得神经元纯度可达 85 %左右 ,培养的第 5～ 7天神经元生长状态最好 ,是神经元形态和机能测试的最佳时期。结论 密度梯度体系离心与抑制有丝分裂相结合可获得纯度较高的脊髓前角神经元。
Objective To provide a reliable and stable method for the isolation and culture of fetal rat spinal cord anterior horn neurons. Methods Primary culture of fetal rat spinal cord anterior horn was performed by density gradient centrifugation with 6. 8% Metri zamide and inhibition of mitosis. Image analysis of NF 2 0 0 immunohistochemistry revealed neuronal growth law. Results The purity of the neurons obtained was about 85%. The cultured neurons grew best on the 5th ~ 7th day, which was the best period for neuronal morphology and function test. Conclusion Centrifugal density gradient system combined with mitosis can be obtained with high purity spinal cord anterior horn neurons.