论文部分内容阅读
为探讨温度胁迫下皱纹盘鲍(Haliotis discus hannai Ino)的响应机制,采用室内控温实验,通过设置4个温度梯度(5℃、10℃、20℃和25℃),设计温度骤变处理组(皱纹盘鲍从15℃暂养温度直接转移至各实验温度)和温度缓变处理组(0.5℃/12 h),分析温度剧烈变化和温度缓慢变化对皱纹盘鲍耗氧率和排氨率的影响及其差异性;并对高温和低温处理下皱纹盘鲍消化腺中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)和溶菌酶(LSZ)活性的变化情况以及不同组织(血细胞和肌肉组织)中Cu/Zn–SOD基因的表达状况进行了研究。结果表明,皱纹盘鲍耗氧率和排氨率随海水温度的升高而增加,20℃达到最高值;25℃骤变处理组与缓变处理组耗氧率和排氨率间存在极显著差异(P<0.01)。5℃和10℃骤变处理组皱纹盘鲍氧氮比(O/N)同缓变处理组间存在极显著差异(P<0.01)。在高温胁迫后的3 h,消化腺中SOD、CAT和LSZ活性达到最高,而ACP活性在胁迫6 h后达到最高(P<0.01);低温胁迫显著降低皱纹盘鲍LSZ的活性,于胁迫9 h后达到最低(P<0.01)。不同温度胁迫下,皱纹盘鲍血细胞和肌肉组织中Cu/Zn–SOD基因的相对表达量均表现上调,与对照组存在显著差异性(P<0.05)。本研究表明,温度胁迫能显著影响皱纹盘鲍的生理和生化活动,这将有助于探讨皱纹盘鲍夏季高死亡率的原因,为皱纹盘鲍健康养殖提供一定的理论依据。
In order to investigate the response mechanism of Haliotis discus hannai Ino under temperature stress, four temperature gradients (5 ℃, 10 ℃, 20 ℃ and 25 ℃) were used to control the temperature. (Abalone Haliotis discus hannai transferred directly from the holding temperature of 15 ℃ to each experimental temperature) and the temperature slowly changing treatment group (0.5 ℃ / 12 h). The effects of the drastic temperature and slow temperature on the oxygen consumption rate and ammonia excretion rate The effects and their differences of the activities of superoxide dismutase (SOD), catalase (CAT), acid phosphatase (ACP) and lysozyme (LSZ) Changes and the expression of Cu / Zn-SOD gene in different tissues (blood cells and muscle tissue) were studied. The results showed that the oxygen consumption rate and ammonia excretion rate of Abalone discus abalone increased with the increase of seawater temperature and reached the highest value at 20 ℃. There was a significant difference between oxygen consumption rate and ammonia excretion rate Difference (P <0.01). There was a significant difference (P <0.01) between the O / N ratio of the abalone discs and the abrupt change treatment groups at 5 ℃ and 10 ℃. At 3 h after heat stress, the activities of SOD, CAT and LSZ in the digestive gland reached the maximum, while the activity of ACP reached the highest level at 6 h after stress (P <0.01). The low temperature stress significantly reduced the activity of LSZ h after reaching the lowest (P <0.01). Under the different temperature stress, the relative expression of Cu / Zn-SOD gene in hamster and muscle tissues of H. discus hannai increased, which was significantly different from the control group (P <0.05). This study shows that temperature stress can significantly affect the physiological and biochemical activities of Haliotis discus hannai, which will help to explore the reasons for the high summer mortality of Haliotis discus hannai and provide a theoretical basis for the healthy culture of Haliotis discus hannai.