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以核质杂种 NC2 1 3 4和 D2 -鉴 2 6(均带有 D2 细胞质 )为细胞质供体 ,以筛选恢复系和转育不育系为目的 ,用 69个春小麦品种为核供体进行回交转育 ,观察不同核质组合后代的育性变化。初步结果表明 ,大约2 8%的品种携带有 D2 细胞质长日光敏雄性不育的恢复基因 ,约 3 5%的品种通过回交可以转育成 D2 细胞质长日光敏雄性不育系。对其中一些组合 F2 群体单株育性的分布情况进行了调查和分析 ,结果表明 ,不同核质组合育性恢复表现不同 ,D2细胞质长日光敏雄性不育育性恢复受 1对或 2对主效基因控制 ,并有一些修饰因子对育性恢复表现有影响。NC2 1 3 4可能携带有 2对 D2细胞质长日光敏雄性不育主效恢复基因 ,而鉴 2 6可能携带1对主效恢复基因。提出了相应的恢复基因遗传假设模式 ,对育性恢复的复杂性进行了讨论
In this study, 69 spring wheat cultivars were used as nucleus donors for NC2134 and D2-Kam26 (both with D2 cytoplasm) as cytoplasmic donors, and for selection of restorer lines and CMS lines Transplantation was conducted to observe the fertility changes of progeny of different nuclear combinations. The preliminary results showed that about 28% of the cultivars carry the restorer gene of D2 cytoplasmic long-day photoperiod male sterile, and about 35% of the varieties can be transferred back to D2 cytoplasmic long-term photoperiod male sterile line by backcrossing. The distribution of single-plant fertility in F2 populations of some combinations was investigated and analyzed. The results showed that the fertility restoration of different combinations of cytoplasm was different. The fertility restoration of D2 cytoplasm in long-day light was affected by one pair or two pairs of main Effective gene control, and some modification factors have an effect on fertility restoration. NC2 1 3 4 may carry two major pairs of D2 cytoplasmic long-term photosensitive male sterility restorer genes, while Kam26 may carry one pair of major effect restorer genes. Put forward the corresponding genetic model of restoring genes and discussed the complexity of fertility restoration