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目的:通过对哮喘大鼠肺泡灌洗液(BALF)中PGD2、IL-5水平及肺功能的检测,探讨Annexin Ⅰ的N-末端肽段Ac2-26在W istar大鼠哮喘模型气道炎症中的作用。方法:清洁级雌性Wiatar大鼠40只随机分为4组,分别为正常对照组(A组),哮喘组(B组),地塞米松治疗组(C组)和Ac2-26治疗组(D组),以卵清蛋白复制大鼠哮喘模型,ELISA法检测肺泡灌洗液中PGD2、IL-5含量,乙酰甲胆碱(MCH)诱发气道高反应性。结果:B组大鼠BALF中PGD2以及IL-5水平均明显高于A组(P<0.01)。C组和D组大鼠BALF中PGD2和IL-5水平均明显低于B组(P<0.01)。C组和D组比较也具有统计学意义。结论:本实验在成功地建立哮喘动物模型基础上,观察到了AC2-26治疗组在改善气道炎症反应作用中较地塞米松治疗组弱,抗炎作用还有待于研究。
OBJECTIVE: To detect the levels of PGD2, IL-5 and pulmonary function in bronchoalveolar lavage fluid (BALF) of asthmatic rats and to investigate the role of Ac2-26, the N-terminal annexin I, in the airway inflammation of Wistar rat asthma model Role. Methods: Forty cleaved female Wiatar rats were randomly divided into four groups: normal control group (group A), asthma group (group B), dexamethasone treatment group (group C) and Ac2-26 treatment group (group D) Group). Ovalbumin was used to replicate the rat asthma model. The content of PGD2 and IL-5 in BALF was detected by ELISA, and airway hyperresponsiveness was induced by methacholine (MCH). Results: The levels of PGD2 and IL-5 in BALF in group B were significantly higher than those in group A (P <0.01). The levels of PGD2 and IL-5 in BALF in group C and group D were significantly lower than those in group B (P <0.01). C group and D group also have statistical significance. Conclusion: Based on the successful establishment of an animal model of asthma, AC2-26 treatment group was observed to be weaker than dexamethasone treatment group in improving the airway inflammatory response. The anti-inflammatory effect remains to be studied.