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目的 探讨胃癌患者外周血T淋巴细胞rDNA转录活性和凋亡相关蛋白变化在肿瘤转移中的意义。方法 采用KL型肿瘤免疫图像分析系统和流式细胞仪测定正常人和胃癌患者外周血T淋巴细胞rDNA的转录活性和凋亡相关蛋白 (Fas/FasL)的表达。结果 胃癌患者外周血T淋巴细胞rDNA转录活性明显降低 ,凋亡相关蛋白Fas表达升高 ,与健康人相比 ,差异显著 (P <0 .0 1) ,随着病情的进展 ,这种趋势更为明显。结论 外周血T淋巴细胞rDNA转录活性和凋亡相关蛋白变化可以从淋巴细胞增殖和凋亡活性两方面评价肿瘤患者的免疫状态 ,在胃癌转移的临床监测中具有重要意义。
Objective To investigate the significance of rDNA transcriptional activities and apoptosis related proteins in peripheral blood T lymphocytes of gastric cancer patients during tumor metastasis. Methods The transcriptional activity of the rDNA and the expression of Fas / FasL protein in T lymphocytes from peripheral blood of normal and gastric cancer patients were determined by KL tumor immunoassay system and flow cytometry. Results The T lymphocyte rDNA transcriptional activity in peripheral blood of patients with gastric cancer was significantly lower than that in healthy individuals (P <0.01), and the trend was more as the disease progressed Obvious. Conclusions The changes of rDNA transcriptional activity and apoptosis related protein in peripheral blood T lymphocytes can evaluate the immune status of tumor patients from two aspects of lymphocyte proliferation and apoptosis activity, which is of great significance in the clinical monitoring of gastric cancer metastasis.