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目的:探讨夏枯草配伍钩藤对自发性高血压大鼠(SHR)血压及心脏的影响。方法:14周龄WKY与SHR大鼠经血压测量后,将10只WKY大鼠作为正常组,50只SHR大鼠依据收缩压差异随机分为模型组、硝苯地平组、夏枯草配钩藤组、夏枯草组、钩藤组,每组10只。正常组和模型组给予等体积的去离子水,硝苯地平组给予硝苯地平缓释片溶液,各中药干预组给予相应剂量的中药浓煎液,连续给药4周。使用Softron BP-98A型大鼠无创尾动脉测压仪测量并记录给药前及给药1、2、3、4周后大鼠尾动脉的收缩压,酶联免疫吸附法(ELISA)测定大鼠血清中ANGⅡ、ET、CGRP的含量,测左心室重量指数,苏木精-伊红染色(HE)观察大鼠心脏形态变化。结果:夏枯草配钩藤组、夏枯草组、钩藤组及硝苯地平组SHR收缩压均低于模型组(P<0.05);硝苯地平组、夏枯草配钩藤组降压效果明显,且夏枯草配钩藤组与硝苯地平组比较,差异无统计学意义(P>0.05);夏枯草配钩藤组的降压效果明显优于夏枯草组与钩藤组。中药干预4周后,夏枯草配钩藤组大鼠血清中ANGⅡ、ET含量及左心室质量指数均低于模型组(P<0.05),CGRP含量高于模型组(P<0.05)。结论:夏枯草配伍钩藤可降低自发性高血压大鼠收缩压,控制由高血压造成的心肌肥厚,其作用机制初步认为与降低血清中ANGⅡ、ET的含量,增加血清中CGRP的含量有关。
Objective: To investigate the effects of Prunella vulgaris with Uncaria rhynchophylla on blood pressure and heart in spontaneously hypertensive rats (SHR). Methods: WKY and SHR rats at 14 weeks of age were measured by blood pressure. Ten WKY rats were selected as the normal group. Fifty SHR rats were randomly divided into model group, nifedipine group, Group, Prunella group, Uncaria group, 10 in each group. The normal group and model group were given equal volume of deionized water, nifedipine group was given nifedipine sustained-release tablets solution, each Chinese medicine intervention group was given the corresponding dose of decoction of Chinese medicine, continuous administration for 4 weeks. The Softron BP-98A rat noninvasive tail arterial pressure manometer was used to measure and record the systolic pressure of the caudal artery in rats before and 1, 2, 3, 4 weeks after administration. The enzyme-linked immunosorbent assay (ELISA) The contents of ANGⅡ, ET and CGRP in rat serum were measured, and the left ventricular mass index and hematoxylin-eosin staining were used to observe the morphological changes of rat heart. Results: Systolic blood pressure (SHR) of Prunella rhynchophylla, Prunella vulgaris, Uncaria rhynchophylla and nifedipine group were significantly lower than those of model group (P <0.05). The antihypertensive effect of nifedipine group and Prunella vulgaris group was significant , And there was no significant difference between Prunella rhynchophylla and Nifedipine group (P> 0.05). The antihypertensive effect of Prunella rhynchophylla with Rhynchophyll group was superior to that of Prunella vulgaris and Uncaria. After intervention for 4 weeks, the content of ANGⅡ, ET and the content of left ventricular mass in the Prunella rhynchophylla group were lower than those in the untreated group (P <0.05). The content of CGRP in the Prunella vulgaris L. group was higher than that in the model group (P <0.05). Conclusion: Prunella compatibility Uncaria can reduce systolic blood pressure in spontaneously hypertensive rats, and control the hypertrophy caused by hypertension. The mechanism of action is preliminarily thought to be related to the reduction of serum levels of ANGⅡ and ET, and the increase of serum CGRP levels.