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目的:研究和探讨食品安全国家标准(GB4789-2010)中食品大肠菌群计数方法,对所用的培养基提出改进意见。方法:按GB4789.3-2010中大肠菌群MPN计数法进行检测。实验分两组:一组为常规月桂基硫酸盐胰蛋白胨肉汤(LST),另一组在月桂基硫酸盐胰蛋白胨肉汤(LST)中加入溴甲酚紫指示剂,其余实验条件均一样。比较两组实验结果。结果:两种培养基的食品大肠菌群计数结果无显著性差异。结论:在月桂基硫酸盐胰蛋白胨肉汤(LST)中加入适量的溴甲酚紫指示剂,对实验结果无影响,但结果观察更直观,清楚。显示本实验的可行性。
Objective: To study and discuss the food coliform counting method in the national standard of food safety (GB4789-2010), and put forward some suggestions for improving the medium used. Methods: According to GB4789.3-2010 coliform MPN count method for detection. The experiment was divided into two groups: one group consisted of conventional lauryl sulfate tryptone broth (LST) and the other group was bromocresol purple indicator in lauryl sulfate tryptose broth (LST). The other experimental conditions were the same . Compare the two experimental results. Results: There was no significant difference in coliform counts between the two media. CONCLUSION: The appropriate amount of bromocresol purple indicator was added to lantosulfate tryptone broth (LST) without any effect on the experimental results, but the results were more intuitive and clear. Show the feasibility of this experiment.