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目的探讨4种饮用水对小鼠肝肾功能及各脏器锌铁含量的影响。方法分别测定纯水、自来水、矿泉水和过滤水中锌、铁浓度。将80只清洁级ICR小鼠随机分为4组,每组20只,雌雄各半,分别用纯水(对照组)、自来水、矿泉水和过滤水喂养,90 d后眼球采血,测定小鼠血清谷丙转氨酶(ALT)、总蛋白(TP)、尿素氮(BUN)、尿酸(UA)及肌酐(Cr)含量,随后立即处死小鼠,取肝脏、肾脏、心脏、胰腺及脑测定锌、铁含量,并制作肝肾组织石蜡切片观察其形态学变化。结果过滤水组小鼠血清Cr含量[(36.00±8.83)μmol/L]明显低于纯水组、自来水组及矿泉水组[(44.83±12.64)、(44.56±10.52)、(43.79±10.53)μmol/L],差异有统计学意义(P<0.05);纯水组小鼠肝脏、肾脏锌含量[(33.17±6.18)、(16.69±8.12)μg/g]明显低于过滤水组[(45.31±7.32)、(22.61±6.01)μg/g],差异有统计学意义(P<0.05);小鼠血清Cr与肾脏锌含量呈显著负相关关系(r=-0.300,P<0.05);不同组小鼠血清ALT、TP、BUN、UA及各脏器内铁含量差异均无统计学意义,同时肝肾组织结构未见异常。结论长期饮用过滤水有利于肾小球滤过屏障功能的发挥,其可能与肾锌水平提高有关。
Objective To investigate the effects of four kinds of drinking water on liver and kidney function and zinc and iron contents in various organs of mice. Methods The concentrations of zinc and iron in pure water, tap water, mineral water and filtered water were determined respectively. Eighty clean-grade ICR mice were randomly divided into four groups, 20 in each group, male and female, respectively, with pure water (control group), tap water, mineral water and filtered water feeding, Serum ALT, TP, BUN, UA and creatinine were measured. Then the mice were sacrificed immediately and the liver, kidneys, heart, pancreas and brain were examined for zinc, Iron content, and making the liver and kidney tissue paraffin sections to observe the morphological changes. Results Compared with pure water group, the content of Cr in serum of filtered water group [(36.00 ± 8.83) μmol / L] was significantly lower than that of pure water group (44.83 ± 12.64, 44.56 ± 10.52, 43.79 ± 10.53, (33.17 ± 6.18) and (16.69 ± 8.12) μg / g] in pure water group were significantly lower than those in the control group [(P <0.05) 45.31 ± 7.32), (22.61 ± 6.01) μg / g], the difference was statistically significant (P <0.05). There was a significant negative correlation between serum Cr and zinc in mice (r = -0.300, P <0.05) The serum ALT, TP, BUN, UA and the contents of iron in different organs of different groups had no statistical significance, meanwhile there was no abnormality in the structure of liver and kidney. Conclusion Long-term drinking filtered water is good for glomerular filtration barrier function, which may be related to renal zinc level.