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目的了解血型糖蛋白Ａ（ＧＰＡ）背景突变水平，观察照射近期的变化规律。方法在正常人和肿瘤放疗病人中进行ＧＰＡ突变分析，结合单克隆抗体标记和流式细胞技术，检测ＭＮ杂合子个体中罕见的变异细胞ＮＯ和ＮＮ，确定变异率。结果对２８个正常成人的初步测定，ＮＯ／ＮＮ变异率（Ｖａｒｉａｎｔｆｒｅｑｕｅｎｃｙ，Ｖｆ）（８．３±４．１３）／（５．５０±３．７７）×１０－６；ＮＮ变异率与年龄相关性接近显著意义（Ｐ＝０．０５７），男女之间、吸烟与不吸烟者均无显著差异。随访肿瘤病人，６例治疗前ＧＰＡ变异率ＮＯ（１２．９±６．６０）×１０－６，ＮＮ（７．７±５．０１）×１０－６，与同年龄者无显著差异。连续盆腔照射１月Ｖｆ已有升高，照射２个月的病人ＮＮＶｆ（１７．５±８．９５）×１０－６，显著高于未照病人（Ｐ＝０．０４６８）。结论正常人ＧＰＡ变异率基本与文献相符，年龄等因素可影响背景水平；病人照射后Ｖｆ变化不一，表明个体对辐射敏感性不同，照射近期主要是ＮＮ的变化，是较晚造血周期细胞突变的表现。
Objective To understand the background level of glycophorin A (GPA) and to observe the recent changes of irradiation. Methods GPA mutation analysis was performed in normal and tumor radiotherapy patients. Monoclonal antibody labeling and flow cytometry were used to detect the rare and differentiated cells NO and NN in MN heterozygous individuals to determine the mutation rate. Results The preliminary determination of 28 normal adults showed that the variability of NO / NN (Vf) was (8.3 ± 4.13) / (5.50 ± 3.77) × 10-6. The mutation rate of NN was correlated with age Sex close to significant (P = 0.057), no significant differences between men and women, smoking and non-smokers. The follow-up of patients with tumor showed that there were no significant differences in the GPA variability between the two groups before treatment (NO (12.9 ± 6.60) × 10-6, NN (7.7 ± 5.01) × 10-6). Vf had been increased in January after continuous pelvic irradiation, and the NNVf (17.5 ± 8.95) × 10-6 in patients who irradiated for 2 months was significantly higher than those in unvaccinated patients (P = 0.0468). Conclusion The variation rate of GPA in normal people is basically consistent with the literature, and age and other factors can influence the background level. Vf changes in patients after irradiation shows that the individuals have different sensitivity to radiation, the change of NN is mainly in the near future, which is the mutation of late hematopoietic cells Performance.