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由美洲商陆(PWM)诱导人脾细胞产生γ干扰素(IFN—γ),再经蓝色琼脂糖CL-6B线性梯度柱层析、一步纯化可将IFN-γ和IL-2洗脱成2个组份。IFN-γ生物活性回收率为770%,比活性达7×10~6IU/mg蛋白.纯化倍数为2682倍.IL-2活性在IFN-γ活性峰前洗出.IFN-γ除在0.5～0.8MNaCl PB洗出一主峰外,用50%乙二醇(EG)+50%IMNaCl也可洗出一个较小的活性峰.由提高离子强度纯化IFN-γ可提示.IFN-γ与蓝色琼脂糖CL-6B是靠离子键结合的.
Interferon-γ (IFN-γ) was induced from human spleen cells induced by Pseudo-American landflower (Pux), and purified by one-step purification with CL-6B linear gradient column chromatography on blue agar to elute IFN-γ and IL-2 2 components. The recovery rate of IFN-γ was 770%, the specific activity was 7 × 10 ~ 6IU / mg protein, the purification fold was 2682. IL-2 activity was eluted before IFN-γ activity peak.IFN- 0.8 MNaCl PB wash out a main peak, with 50% ethylene glycol (EG) + 50% IMNaCl also wash out a smaller peak activity by increasing the ionic strength of purified IFN-γ can be prompted.IFN-γ and blue Sepharose CL-6B is ionically bound.