低温、高盐和干旱胁迫严重影响植物的生长和产量。本研究以花生品种花育33号为实验材料,根据cDNA文库中已知的蔗糖合成酶基因AhSuSy全长序列设计引物,通过RT-PCR克隆到该基因。通过荧光定量PCR分析了该基因在花生各组织中的表达及在低温、高盐等非生物胁迫下的表达。结果显示,该基因为组成型表达基因,在叶片和根中表达量较高,在花中表达量最低;AhSuSy基因在花生的叶片和根中对低温均没有明显响应,但在花生根中受高盐胁迫和干旱胁迫明显诱导,说明该基因可能参与了花生对高盐和干旱胁迫的适应性调控;AhSuSy在花生根中受ABA的明显诱导,说明该基因对花生非生物胁迫的调控可能是以依赖ABA的方式进行的。 Low temperature, high salt and drought stress seriously affect plant growth and yield. In this study, Huayu 33, a peanut variety, was used as the experimental material. Based on the full-length sequence of the AhSuSy sucrose synthase gene in the cDNA library, primers were designed and cloned by RT-PCR. The expression of this gene in various tissues of peanut and its expression under abiotic stress such as low temperature and high salt were analyzed by fluorescence quantitative PCR. The results showed that the gene was a constitutively expressed gene and expressed higher in leaves and roots and lowest in flowers. AhSuSy gene had no obvious response to low temperature in the leaves and roots of peanut, but was negatively affected by peanut roots High salt stress and drought stress were significantly induced, indicating that the gene may be involved in the adaptive regulation of peanut to high salt and drought stress; AhSuSy significantly induced by ABA in root of peanut, indicating that the gene regulation of peanut abiotic stress may be In a way that depends on ABA.