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为明确洛阳市牡丹灰霉病的病原菌种类,于2015年从该地区的4个牡丹Paeonia suffruticosa Andr.种植区采集灰霉病样品,采用组织分离法对病原菌进行分离和纯化,结合形态学和基因序列分析对分离物进行鉴定,采用针刺接种法测定不同分离物对牡丹离体叶片的致病性。结果表明,从灰霉病样品中分离获得40株分离物,这些分离物分别属于Ⅰ、Ⅱ、Ⅲ型菌,I型菌不易产孢但产菌核;Ⅱ型菌易产孢,后期产生少量菌核;Ⅲ型菌易产孢,且产生大量菌核。产孢分离物均形成直立状分生孢子梗,孢子梗分枝顶端聚生葡萄穗状分生孢子,分生孢子卵圆形或长卵圆形。供试菌株的ITS序列和G3PDH基因序列与灰葡萄孢Botrytis cinerea Pers.的同源性达到99%;致病性测定结果表明,各菌群代表菌株对牡丹均有致病性,但不同菌群间致病力有差异。研究表明,引起洛阳市牡丹灰霉病的病原菌为灰葡萄孢,且菌群类型多样。
In order to clarify the pathogen species of Peony gray mold in Luoyang City, gray mold samples were collected from 4 peony plantations of Paeonia suffruticosa Andr. In the area in 2015. The pathogenic bacteria were isolated and purified by tissue isolation method. Morphological and gene Sequence analysis was used to identify the isolates. The pathogenicity of different isolates to detached leaves of Peonies was determined by acupuncture inoculation. The results showed that 40 isolates were isolated from the samples of Botrytis cinerea, which belonged to type I, II and III, respectively. Type I bacteria did not produce spores but produced bacteria nucleus. Sclerotium; Type Ⅲ bacteria easy sporulation, and produce a large number of sclerotia. Spore isolates formed upright conidiophores, conidiophores spike conidia, conidia oval or oblong. The homology of ITS sequence and G3PDH gene sequence of the tested strains to Botrytis cinerea Pers. Reached 99%. The pathogenicity test results showed that all the strains showed pathogenicity to Peony, Pathogenicity between the differences. Studies have shown that the pathogen caused by Botrytis cinerea Luoyang City is Botrytis cinerea, and the flora is diverse.