由于致倦库蚊染色体长而脆,分离困难,采用以往的方法很难获得完整清晰的多线染色体标本。本文作者介绍了一种简单、重现性好而且展开得较好的获自致倦库蚊马氏管的多线染色体方法。 实验用的致倦库蚊雌蚊采自印度加尔各答郊区。在pH7.3的磷酸缓冲液中,将马氏管从吸血成蚊体内解剖出来,移置于一滴新 Due to the long and brittle chromosomes of Culex quinquefasciatus and the difficulty of separation, it is difficult to obtain complete and clear multiline chromosome samples by using the previous methods. The authors describe a simple, reproducible, and well-developed multilineage chromosome approach obtained from M. cuvatis. The experimental Ctriplex mosquitoes were collected from the suburbs of Calcutta, India. In pH7.3 phosphate buffer, the tube was dissected from a blood-sucking mosquito and placed in a drop of fresh