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对来源于兰州和上海两市的两株腮腺炎病毒野毒的小疏水蛋白（ＳＨ）基因及其旁侧区ｃＤＮＡ的ＰＣＲ扩增产物，分别进行直接测序和克隆测序。用直接测序法测定２株腮腺炎野毒３７８个核苷酸序列，在此范围内存在１６个核苷酸（４．２％）差异，其中１１个（２．９％）位于编码区序列中，所推导的ＳＨ蛋白序列有６个氨基酸（１０．５％）差异。克隆测序法测定的这两株腮腺炎野毒的核苷酸序列相当于直接法的７８－３７８位核苷酸。两种方法在所测３０１个核苷酸（ｎｔ）的共同区域内序列完全一致。证明ＰＣＲ产物直接测序用于腮腺炎病毒分子流行病学研究不仅特异性强，敏感度高，而且比克隆测序法更为快速简便，节省材料
The two wild mumps-derived small hydrophobin (SH) genes and their flanking region PCR products from Lanzhou and Shanghai were directly sequenced and cloned separately. Two mtDNA wild-type toxin 378 nucleotide sequences were sequenced by direct sequencing. Within this range, there were 16 nucleotide (4.2%) differences, of which 11 (2.9%) were located in the coding region , The deduced SH protein sequence has 6 amino acids (10.5%) difference. The nucleotide sequences of these two mumps wild-type toxins, as determined by cloning and sequencing, corresponded to nucleotides 78-378 of the direct method. The two methods are exactly the same in the common region of 301 nucleotides (nt) measured. Demonstrating Direct Sequencing of PCR Products for Mumps Molecular epidemiology is not only specific and sensitive, it is faster and easier than cloning and sequencing, and saves material