Objective: To observe the effect of tonifying Kidney herbs (TKH) on pituitary gland, ovary,and adrenal gland of androgen sterilized rats (ASR). Methods: ASR model was established by injecting testosterone propionate subcutaneously to Sprague-Dawley (SD) female rats of 9 days age. Morphological and endocrinological change of pituitary gland, ovary and adrenal gland in rats of 100 - 107 days old before and after feeding TKH extract were observed by light and electron microscope, cell cultures, immunohistochemical studies, and radioimmunoassay. Results: In the ASR groups, there were intracytoplasmic lipid drops, crinophagy,vacuole, and autophagy in pituitary gland, anovulation in ovary, and hyperplasia of reticular zone in adrenal gland. At the same time, serum follicular stimulating hormone and luteinizing hormone decreased (P < 0. 05-0. 005) and serum testosterone and dehydroepiandrosterone levels increased significantly (P < 0. 0 1- 0. 0 0 1 ).These morphologiacl and endocrinological changes were all returned to normal range after the TKH treatment.Conclusions: The sex gland axis and adrenal gland of SD rats of 9 days old were disturbed after exogenous androgen treating, which played an important role in pathogenesis of ASR, TKH could reduce androgen level and induce ovulation through regulating sex gland axis and adrenal gland in multi-levels and multi-target organs. Objective: To observe the effect of tonifying Kidney herbs (TKH) on pituitary gland, ovary, and adrenal gland of androgen sterilized rats (ASR). Methods: ASR model was established by injecting testosterone propionate subcutaneously to Sprague-Dawley of 9 days old. Morphological and endocrinological changes of pituitary gland, ovary and adrenal gland in rats of 100 - 107 days old before and after feeding TKH extract were observed by light and electron microscope, cell cultures, immunohistochemical studies, and radioimmunoassay. Results: In the ASR groups, there were intracytoplasmic lipid drops, crinophagy, vacuole, and autophagy in pituitary gland, anovulation in ovary, and hyperplasia of reticular zone in adrenal gland. At the same time, serum follicular stimulating hormone and luteinizing hormone decreased (P < 0. 05-0. 005) and serum testosterone and dehydroepiandrosterone levels increased significantly (P <0. 0 1- 0. 0 0 1). These morphologiacl and endocrinological c Hanges were all returned to normal range after the TKH treatment. Confc: The sex gland axis and adrenal gland of SD rats of 9 days old were disturbed after exogenous androgen treating, which played an important role in pathogenesis of ASR, TKH could reduce androgen level and induce ovulation through regulating sex gland axis and adrenal gland in multi-levels and multi-target organs.