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为评估转基因抗虫棉花对天敌的安全性,用取食常规棉(中棉所49)、单价棉(中棉所45)和双价棉(2009002)的棉铃虫幼虫饲喂草间小黑蛛,采用酶联免疫和分光光度计法研究转基因抗虫棉对草间小黑蛛生长发育及其相关酶活性的影响。结果显示,与常规棉花相比,单价棉花使草间小黑蛛4～5龄幼虫发育历期显著延长,对其余龄期影响不显著;双价棉花对草间小黑蛛1～3龄幼虫发育历期影响不显著,4～5龄幼虫发育历期显著延长,6龄后大量死亡。2种转基因棉对草间小黑蛛体重及其体内外源蛋白含量的影响均不显著,除谷胱甘肽-转移酶和单价棉处理的过氧化物酶外,对其它相关酶活性亦无显著影响。
In order to assess the safety of transgenic insect-resistant cotton to natural enemies, black spider moths were fed on cotton bollworm larvae fed conventional cotton (CIMC 49), monovalent cotton (CIMC 45) and cotton (2009002) , The effects of transgenic Bt cotton on the growth and development and the related enzyme activities of Spartina alterniflora were studied by enzyme - linked immunosorbent assay and spectrophotometer. The results showed that compared with the conventional cotton, the monospecific cotton prolonged the developmental duration of 4th to 5th instar larvae, but had no significant effect on the rest of the larvae. The developmental duration had no significant effect, the developmental duration of 4th to 5th instar larvae significantly prolonged, and a large number of deaths occurred after the sixth instar larvae. The effects of two kinds of transgenic cotton on body weight and exogenous protein content were not significant. Except glutathione-transferase and peroxidase treated with monovalent cotton, the activities of other related enzymes Significantly affected.