Goosegrass is a worst grass weed in orchards and turf.The increased use of glyphosate for goosegrass control has led to the occurrence of many resistant populations.Although glyphosate has been used to control weeds for the past 30 years in China,few reports are available on glyphosate-resistant(GR) googegrass.In this study,we determined the GR level of 14 goosegrass populations from Chengdu and Guangzhou,China.Glyphosate only controlled 3.1 and 25.0%of the populations SL5 and SL1,respectively,at the dose of 1 680 g acid equivalent(ae) ha~(-1) at 14 days after treatment(DAT).In contrast,the susceptible population(XD1) was completely(100%) controlled.The resistant index(Rl) of SL5 and SL1 were 5.1 and 4.5,and the Rl for SL2,SL3 and ZC1 were 4.2,3.2 and 2.6,respectively.The Rl for other populations was range from 1.8 to 2.5.Under the dose of glyphosate at 1 640 g ae ha~(-1) at 10 DAT,shikimate accumulation in susceptible population XD1 was 17.6 and 16.4 times higher than SL5 and SL1,respectively.And the chlorophyll content in the plant leaf of populations SL1,SL2 and SL5 were decreased slightly ranging from 22.6 to 28.0.These results confirmed that the SL1,SL2,SL3,ZC1 and SL5 populations had evolved moderate resistance to glyphosate.This is the first report for the GR goosegrass populations confirmed in Chengdu,China. Goosegrass is a worst grass weed in orchards and turf. The increased use of glyphosate for goosegrass control has led to the occurrence of many resistant populations. Though glyphosate has been used to control weeds for the past 30 years in China, few reports are available on glyphosate-resistant (GR) googegrass.In this study, we determined the GR level of 14 goosegrass populations from Chengdu and Guangzhou, China. Glyphosate only controlled 3.1 and 25.0% of the populations SL5 and SL1, respectively, at the dose of 1 680 In vivo, the susceptible population (XD1) was completely (100%) controlled. The resistant index (Rl) of SL5 and SL1 were 5.1 and 4.5, and the Rl for SL2, SL3 and ZC1 were 4.2, 3.2 and 2.6, respectively. The Rl for other populations was range from 1.8 to 2.5. Unit dose of glyphosate at 1 640 g ae ha -1 (at) 10 DAT, shikimate accumulation in susceptible population XD1 was 17.6 and 16.4 times higher than SL5 and SL1, resp The results of the SL1, SL2, SL3, ZC1 and SL5 populations had evolved moderate resistance to glyphosate. this is the first report for the GR goosegrad surely confirmed in Chengdu, China.