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3T3-L1前脂肪细胞从17～19日龄小鼠胚胎Swiss3T3细胞中分离获得,其具有单一分化潜能,可通过细胞增殖过程达到细胞生长抑制,从生长周期退出的细胞经诱导激素诱导,开始出现脂肪细胞的表型,细胞内三酰甘油(TG)开始积聚,最终分化成为成熟的脂肪细胞。3T3-L1前脂肪细胞是目前前脂肪细胞增殖、向脂肪细胞分化研究领域应用最广泛的细胞系之一,常用于前脂肪细胞分化过程中基因表达调
3T3-L1 preadipocytes were isolated from Swiss3T3 cells of 17-19 day-old mouse embryos, which had single differentiation potential and reached the cell growth inhibition through the process of cell proliferation. The cells exiting from the growth cycle were induced by induction hormone and began to appear The phenotype of adipocytes, intracellular triglycerides (TGs) begin to accumulate and eventually differentiate into mature adipocytes. 3T3-L1 preadipocytes is one of the most widely used cell lines in the field of adipocyte differentiation, and is commonly used in the process of preadipocyte differentiation and gene expression